File(s) not publicly available
Spectral and dose dependence of light-induced ion flux responses from maize leaves and their involvement in leaf expansion growth
journal contributionposted on 2023-05-16, 20:27 authored by Branka Zivanovic, Tracey Cuin, Sergey ShabalaSergey Shabala
Two types of segments (intact leaf tissue and isolated mesophyll tissue respectively) were isolated from basal (still growing) and tip (non-growing) maize leaf regions. The leaf segments were exposed to different light qualities (blue or red light) and quantities, and net fluxes of K+, Ca 2+ and H+ were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). A clear dose dependency of all ion flux responses on both red (RL) and blue (BL) light fluence rate was found. We provide evidence that light-induced K+ flux kinetics are different between growing and non-growing tissues and attribute this difference to the direct involvement of RL-induced K+ flux in turgor-driven leaf expansion growth controlled by the epidermis, as well as to the charge-balancing role of K+ in the leaf mesophyll. Generally, BL was much more efficient in stimulating K+ uptake in the growing basal region compared with RL. We also show a much stronger influence of RL on Ca 2+ fluxes in the basal region compared with BL, which argues in favor of the importance of RL in Ca2+ signaling during leaf growth. Â© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
Publication titlePlant and Cell Physiology
Department/SchoolTasmanian Institute of Agriculture (TIA)
PublisherOxford University Press
Place of publicationUnited Kingdom, Japan