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144892 - Stages of embryonic development in the live-bearing fish.pdf (57.6 MB)

Stages of embryonic development in the live-bearing fish, Gambusia holbrooki

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journal contribution
posted on 2023-05-21, 00:01 authored by Seyed Ehsan MousaviSeyed Ehsan Mousavi, Jawahar PATILJawahar PATIL

Background

Divergent morphology and placentation of Poeciliids make them suitable model for investigating how evolutionary selection has altered and conserved the developmental mechanisms. However, there is limited description of their embryonic staging, despite representing a key evolutionary node that shares developmental strategy with placental vertebrates. Here we describe the embryonic developmental stages of Gambusia holbrooki from zygote to parturition using freshly harvested embryos.

Results

We defined 40 embryonic stages using a numbered (stages 0-39; zygote to parturition respectively) and named (grouped into seven periods that is, zygote, cleavage, blastula, gastrula, segmentation, pharyngula and parturition) staging system. Two sets of quantitative (ie, egg diameter, embryonic total length, otic vesicle closure index, heart rates, the number of caudal fin rays and elements) and qualitative (ie, three-dimensional analysis of images and key morphological criteria) data were acquired and used in combination to describe each stage. All 40 stages are separated by well-defined morphological traits, revealing developmental novelties that are influenced by narrow perivitelline space, placentation, internal gestation, and sex differentiation.

Conclusions

The principal diagnostic features described are quick, reliable, and easy to apply. This system will benefit researchers investigating molecular ontogeny, particularly sexual differentiation mechanisms in G. holbrooki.

History

Publication title

Developmental Dynamics

Volume

251

Pagination

287-320

ISSN

1097-0177

Department/School

Institute for Marine and Antarctic Studies

Publisher

John Wiley & Sons Inc

Place of publication

United States

Rights statement

Copyright 2021 American Association for Anatomy

Repository Status

  • Restricted

Socio-economic Objectives

Control of pests, diseases and exotic species in coastal and estuarine environments; Climate change models