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Study of New Extraction Methods for Separation of Anthocyanins from Red Grape Skins: Analysis by HPLC and LC-MS/MS
journal contribution
posted on 2023-05-16, 22:25 authored by Ghassempour, A, Heydari, R, Talebpour, Z, Fakhari, AR, Rassouli, A, Noel DaviesNoel Davies, Aboul-Enein, HYMicrowave assisted extraction (MAE) and ultrasound assisted extraction (UAE) methods were applied for the first time for the extraction of anthocyanins from red grape skins, and the results of these methods were compared with the maceration method. The extracted samples were analyzed by high performance liquid chromatography (HPLC) and nine anthocyanins were identified by liquid chromatography tandem mass spectrometry (LC-MS=MS). The effects of sample weight to solvent volume ratio, temperature, and time of extraction on the efficiency of extraction in MAE were investigated by two full factorial designs at three levels. The results of two designs show that sample weight to solvent volume ratio and temperature were more important than the time of extraction in MAE. UAE has no variable parameters similar to MAE. The suitable solvent and required time for the UAE method were described. The recoveries of MAE, UAE, and maceration methods were 90%, 82%, and 98%, respectively.
History
Publication title
Journal of Liquid Chromatography & Related TechnologiesVolume
31Issue
17Pagination
2686-2703ISSN
1082-6076Publisher
Taylor & FrancisPlace of publication
PhiladelphiaRights statement
The definitive published version is available online at: http://www.tandf.co.uk/journalsRepository Status
- Restricted