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Surface Plasmon Resonance Spectroscopy: A New Lead in Studying the Membrane Binding of Amyloidogenic Transthyretin

journal contribution
posted on 2023-05-17, 10:47 authored by Hou, X, David SmallDavid Small, Aguilar, MI
Surface plasmon resonance (SPR) employs the optical principle of SPR to measure changes in mass on a sensor chip surface in real time. Surface chemistry has been developed which enables the immoblization of lipid bilayers and determination of protein-membrane interactions in real time. In the last decade, the plasma membrane has been demonstrated to play an important role in amyloidogenesis and cytotoxicity induced by amyloidogenic proteins. SPR provides an ideal way to study the membrane binding of amyloidogenic proteins. In this chapter, we describe the application of SPR to the study of amyloidogenic transthyretin binding to the plasma membrane and artificial lipid bilayers.

History

Publication title

Methods in Molecular Biology

Volume

752

Pagination

215-228

ISSN

1064-3745

Department/School

Menzies Institute for Medical Research

Publisher

Humana Press, Inc.

Place of publication

United States

Rights statement

Copyright 2011 Springer Science+Business Media, LLC

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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