Testosterone Determination Using Rapid Heterogeneous Competitive-Binding for Enzyme-Linked Immunosorbent Assay in Flow Injection

The application of a rapid competitive enzyme-linked immunosorbent assay (ELISA) for the measurement of testosterone in flow injection (FI) is presented. The ELISA protocol for the determination of testosterone was performed in 60 min compared to the standard protocol time of 150 min. This was achieved by reducing the antibody incubation time. The ELISA was used successfully to determine testosterone levels in clinical samples of both male and female subjects, and were in good agreement compared to a pathology laboratory which employed an immunoradiometric assay (IRMA).