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The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels
journal contributionposted on 2023-05-19, 10:58 authored by Hwang, E-S, Cao, C, Hong, S, Jung, H-J, Cha, C-Y, Choi, J-B, Kim, Y-J, Baik, S
Single walled carbon nanotubes (SWNTs) exhibit strong Raman signals as well as fluorescence emissions in the near infrared region. Such signals do not blink or photobleach under prolonged excitation, which is an advantage in optical nano-biomarker applications. In this paper, we present single-stranded DNA conjugated SWNT probes to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblasts and Escherichia coli are used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrates excellent sensitivity and specificity of the probes. The results show that SWNTs may be used as generic nano-biomarkers for the precise detection of specific kinds of genes.
Department/SchoolSchool of Natural Sciences
PublisherIop Publishing Ltd
Place of publicationDirac House, Temple Back, Bristol, England, Bs1 6Be
Rights statementCopyright 2006 IOP Publishing Ltd.