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The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury

journal contribution
posted on 2023-05-17, 19:09 authored by Gonzalez, P, Carmen Fernandez-MartosCarmen Fernandez-Martos, Arenas, E, Rodriguez, FJ
Wnt proteins play a critical role in central nervous system development and have been implicated in several neuropathologies, including spinal cord injury (SCI). Ryk, an unconventional Wnt receptor, regulates axonal regeneration after SCI, although its expression pattern in this neuropathology remains unclear. Therefore, we sought to define the spatiotemporal and cellular pattern of Ryk expression after a contusive SCI in adult rats using quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemical analysis. Under physiological conditions, Ryk is expressed in neurons, astrocytes, and blood vessels, but not in oligodendrocytes, microglia, NG2+ glial precursor cells, or axonal projections. Following SCI, we observed an increase in Ryk mRNA expression from 24 h post-injury until 7 days post-injury, whereas its protein levels were significantly augmented at 7 and 14 days post-injury. Moreover, the spatial and cellular Ryk expression pattern was altered in the damaged tissue, where this receptor was observed in reactive astrocytes and microglia/macrophages, NG2+ glial precursors, fibronectin+ cells, oligodendrocytes, and axons. In conclusion, we demonstrate that Ryk is expressed in the unlesioned spinal cord and that, after SCI, its spatiotemporal and cellular expression pattern changed dramatically, being expressed in cells involved in the spinal cord response to damage.

History

Publication title

Journal of Neurotrauma

Volume

30

Issue

10

Pagination

806 - 817

ISSN

0897-7151

Department/School

Wicking Dementia Research Education Centre

Publisher

Mary Ann Liebert Inc Publ

Place of publication

2 Madison Avenue, Larchmont, USA, Ny, 10538

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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