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The development of a fluorescent antibody stain to identify a Uronema sp. (Ciliophora:Scuticociliatida) implicated in fatal encephalitis in southern bluefin tuna (Thunnus Maccoyii)
journal contribution
posted on 2023-05-16, 10:08 authored by Watts, M, Burke, CM, Munday, BLPolyclonal antisera, designated anti-Q antiserum and anti-B antiserum, were produced in rabbits against Uronema sp. isolated from diseased tuna (Q) and dead blenny larvae (B). Extraneous bacterial antibodies and cross-reacting ciliate antibodies were present in the antiserum and were removed by adsorption, enabling Uronema sp. to be easily differentiated within a marine ciliate assemblage. Adsorbed anti-B antiserum, diluted to 1/50, was used in indirect fluorescent antibody tests (IFATs) and detected ≥ 10 Uronema sp. in a 1 L sample of seawater and approximately 10 presumptive Uronema/15 μL in sediment samples from a tuna sea-cage. In addition, a variable staining pattern was demonstrated with different isolates of Uronema sp. The results indicate that this test is able to detect both cultured and environmental Uronema sp. and may have an application both as an environmental monitoring tool and a means of speciating Uronema.
History
Publication title
Bulletin of the European Association of Fish PathologistsVolume
16Pagination
104-108ISSN
0108-0288Department/School
School of Health SciencesPublisher
Eur Assoc Fish PathologistsPlace of publication
C/O Dr David Bruno, Marine Laboratory, Po Box 101, Victoria Rd, Aberdeen, Scotland, Ab11 9DbRepository Status
- Restricted