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The gibberellin status of lip1, a mutant of Pisum sativum L. which exhibits light-independent photomorphogenesi
journal contributionposted on 2023-05-16, 10:06 authored by Sponsel, VM, John RossJohn Ross, Reynolds, MR, Symons, GM, James ReidJames Reid
Dark-grown seedlings of the lip1 (light independent photomorphogenesis) mutant of Pisum sativum L. display many features of de-etiolated growth and are similar in many respects to wild-type (WT) seedlings grown in the light. The involvement of gibberellins (GAs) with the mutant phenotype was examined by applying GA1 and GA20 to the mutant and WT, and by quantifying endogenous GA1, GA8, GA19, GA20, and GA29 levels in the two genotypes. These experiments were conducted in both the light and the dark. In neither environment could GA application restore elongation in the mutant to that in GA-treated WT plants. Quantification of GAs provided further evidence that the mutant phenotype is not attributable to a deficiency in endogenous GA1. However, dark-grown lip1 seedlings contained lower levels of GA19 and higher levels of GA20 than dark-grown WT plants, whereas in the light, the effect of the mutation on the ratio of GA19 to GA20 was reversed. Thus, there appears to be a complex interaction between the lip1 mutation, the light regime, and the step GA19 to GA20.
Publication titlePlant Physiology
Department/SchoolSchool of Natural Sciences
PublisherAmer Soc Plant Biologists
Place of publication15501 Monona Drive, Rockville, USA, Md, 20855