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The kinetics and regulation of phosphofructokinase from Teladorsagia circumcincta
journal contribution
posted on 2023-05-17, 15:45 authored by Walker, LR, Simcock, DC, Pedley, KC, Simpson, HV, Brown, SPhosphofructokinase (PFK-1) activity was examined in L 3 and adult Teladorsagia circumcincta, both of which exhibit oxygen consumption. Although activities were higher in the adult stage, the kinetic properties of the enzyme were similar in both life cycle stages. T. circumcincta PFK-1 was subject to allosteric inhibition by high ATP concentration, which increased both the Hill coefficient (from 1.4±0.2 to 1.7±0.2 in L 3s and 2.0±0.3 to 2.4±0.4 in adults) and the K 1/2 for fructose 6 phosphate (from 0.35±0.02 to 0.75±0.05mM in L 3s and 0.40±0.03 to 0.65±0.05mM in adults). The inhibitory effects of high ATP concentration could be reversed by fructose 2,6 bisphosphate and AMP, but glucose 1,6 bisphosphate had no effect on activity. Similarly, phosphoenolpyruvate had no effect on activity, while citrate, isocitrate and malate exerted mild inhibitory effects, but only at concentrations exceeding 2mM. The observed kinetic properties for T. circumcincta PFK-1 were very similar to those reported for purified Ascaris suum PFK-1, though slight differences in sensitivity to ATP concentration suggests there may be subtle variations at the active site. These results are consistent with the conservation of properties of PFK-1 amongst nematode species, despite between species variation in the ability to utilise oxygen. © 2012 Elsevier Inc.
History
Publication title
Experimental ParasitologyVolume
130Issue
4Pagination
348-353ISSN
0014-4894Department/School
School of Health SciencesPublisher
Academic PressPlace of publication
3251 Riverport Ln, Maryland Heights, MO 63043, USARights statement
Copyright 2012 2012 Elsevier Inc.Repository Status
- Restricted
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