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The metabolome of Chlamydomonas reinhardtii following induction of anaerobic H2 production by sulfur depletion
journal contributionposted on 2023-05-18, 11:17 authored by Matthew, T, Zhou, W, Rupprecht, J, Lim, L, Thomas-Hall, SR, Doebbe, A, Kruse, O, Hankamer, B, Marx, UC, Steven SmithSteven Smith, Schenk, PM
The metabolome of the model species Chlamydomonas reinhardtii has been analyzed during 120 h of sulfur depletion to induce anaerobic hydrogen (H2) production, using NMR spectroscopy, gas chromatography coupled to mass spectrometry, and TLC. The results indicate that these unicellular green algae consume freshly supplied acetate in the medium to accumulate energy reserves during the first 24 h of sulfur depletion. In addition to the previously reported accumulation of starch, large amounts of triacylglycerides were deposited in the cells. During the early 24- to 72-h time period fermentative energy metabolism lowered the pH, H2 was produced, and amino acid levels generally increased. In the final phase from 72 to 120 h, metabolism slowed down leading to a stabilization of pH, even though some starch and most triacylglycerides remained. We conclude that H2 production does not slow down due to depletion of energy reserves but rather due to loss of essential functions resulting from sulfur depletion or due to a build-up of the toxic fermentative products formate and ethanol.
Publication titleJournal of Biological Chemistry
Department/SchoolSchool of Natural Sciences
PublisherAmer Soc Biochemistry Molecular Biology Inc
Place of publication9650 Rockville Pike, Bethesda, USA, Md, 20814-3996
Rights statement© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.