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Download fileThe reproducibility of 31-phosphorous MRS measures of muscle energetics at 3 tesla in trained men
journal contribution
posted on 2023-05-17, 14:25 authored by Edwards, LM, Tyler, DJ, Kemp, GJ, Renee RossRenee Ross, Johnson, A, Holloway, CJ, Nevill, AM, Clarke, KObjective Magnetic resonance spectroscopy (MRS) provides an exceptional opportunity for the study of in vivo metabolism. MRS is widely used to measure phosphorus metabolites in trained muscle, although there are no published data regarding its reproducibility in this specialized cohort. Thus, the aim of this study was to assess the reproducibility of 31P-MRS in trained skeletal muscle. Methods We recruited fifteen trained men (VO2peak = 4.7±0.8 L min−1/58±8 mL kg−1 min−1) and performed duplicate MR experiments during plantar flexion exercise, three weeks apart. Results Measures of resting phosphorus metabolites were reproducible, with 1.7 mM the smallest detectable difference in phosphocreatine (PCr). Measures of metabolites during exercise were less reliable: exercising PCr had a coefficient of variation (CV) of 27% during exercise, compared with 8% at rest. Estimates of mitochondrial function were variable, but experimentally useful. The CV of PCr1/2t was 40%, yet much of this variance was inter-subject such that differences of <20% were detectable with n = 15, given a significance threshold of p<0.05. Conclusions 31-phosphorus MRS provides reproducible and experimentally useful measures of phosphorus metabolites and mitochondrial function in trained human skeletal muscle.
History
Publication title
PLoS OneVolume
7Issue
6Article number
e37237Number
e37237Pagination
1-7ISSN
1932-6203Department/School
Tasmanian School of MedicinePublisher
Public Library of SciencePlace of publication
1160 Battery St, San Francisco, CA 94111, USARights statement
Licensed under Creative Commons Attribution 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/Repository Status
- Open