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The role of strigolactones in photomorphogenesis of pea is limited to adventitious rooting

journal contribution
posted on 2023-05-18, 02:47 authored by Shelley UrquhartShelley Urquhart, Eloise FooEloise Foo, James ReidJames Reid
The recently discovered group of plant hormones, the strigolactones, have been implicated in regulating photomorphogenesis. We examined this extensively in our strigolactone synthesis and response mutants and could find no evidence to support a major role for strigolactone signaling in classic seedling photomorphogenesis (e.g. elongation and leaf expansion) in pea (Pisum sativum), consistent with two recent independent reports in Arabidopsis. However, we did find a novel effect of strigolactones on adventitious rooting in darkness. Strigolactone-deficient mutants, Psccd8 and Psccd7, produced significantly fewer adventitious roots than comparable wild-type seedlings when grown in the dark, but not when grown in the light. This observation in dark-grown plants did not appear to be due to indirect effects of other factors (e.g. humidity) as the constitutively de-etiolated mutant, lip1, also displayed reduced rooting in the dark. This role for strigolactones did not involve the MAX2 F-Box strigolactone response pathway as Psmax2 f-box mutants did not show a reduction in adventitious rooting in the dark compared with wild-type plants. The auxin-deficient mutant bushy also reduced adventitious rooting in the dark, as did decapitation of wild-type plants. Rooting was restored by the application of indole-3-acetic acid (IAA) to decapitated plants, suggesting a role for auxin in the rooting response. However, auxin measurements showed no accumulation of IAA in the epicotyls of wild-type plants compared with the strigolactone synthesis mutant Psccd8, suggesting that changes in the gross auxin level in the epicotyl are not mediating this response to strigolactone deficiency.


Australian Research Council


Publication title

Physiologia Plantarum








School of Natural Sciences


Blackwell Munksgaard

Place of publication

35 Norre Sogade, Po Box 2148, Copenhagen, Denmark, Dk-1016

Rights statement

Copyright 2014 Scandinavian Plant Physiology Society

Repository Status

  • Restricted

Socio-economic Objectives

Environmentally sustainable plant production not elsewhere classified

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