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The use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms

journal contribution
posted on 2023-05-16, 20:36 authored by Parry, JD, Holmes, AK, Unwin, ME, Laybourn-Parry, J
Aims: This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Methods and Results: Coupons of dialysis membrane were incubated in Chalkley’s medium for 3 days at 23!C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. Conclusions: The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Significance and Impact of the Study: Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.

History

Publication title

Letters in Applied Microbiology

Volume

45

Issue

4

Pagination

364-370

ISSN

0266-8254

Publisher

Blackwell Publishing Ltd

Place of publication

United Kingdom

Rights statement

The definitive published version is available online at: http://interscience.wiley.com

Repository Status

  • Restricted

Socio-economic Objectives

Biodiversity in Antarctic and Southern Ocean environments

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