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Thread-based isotachophoresis coupled with desorption electrospray ionization mass spectrometry for clean-up, preconcentration, and determination of alkaloids in biological fluids
journal contributionposted on 2023-05-21, 02:21 authored by Liang ChenLiang Chen, Alireza GhiasvandAlireza Ghiasvand, Lam, SC, Estrella Sanz RodriguezEstrella Sanz Rodriguez, Innes, PC, Brett PaullBrett Paull
A thread-based isotachophoresis method coupled with desorption electrospray ionization mass spectrometry (TB-ITP-DESI-MS) was developed and applied for clean-up, preconcentration, and determination of alkaloids in biological fluids. This simple approach enables the focusing and rapid analysis of analytes of interest in complex matrices that are otherwise challenging using direct ambient mass spectrometry. The TB-ITP platform components were rapidly and reproducibly fabricated at low-cost using 3D printing. A single string of nylon 6 thread was used as the electrophoresis substrate and a cotton knot, tied to the nylon thread, was used as the trapping zone of the ITP focused model analytes (coptisine, berberine and palmatine). The trapping efficiency was evaluated upon different commercially available threads with different chemical properties and cotton was selected as the best material due to its highest trapping efficiency and subsequent DESI-MS ionization efficiency. Up to 11.6-fold increase in signal to noise ratio (S/N) was obtained using the proposed method compared to direct DESI-MS detection, due to the reduced matrix interference and focusing. The results demonstrated that the TB-ITP-DESI-MS approach is a viable solution for the analysis of complicated biological fluid samples.
Publication titleAnalytica Chimica Acta
Department/SchoolSchool of Natural Sciences
PublisherElsevier Science Bv
Place of publicationPo Box 211, Amsterdam, Netherlands, 1000 Ae
Rights statement© 2021 Elsevier B.V. All rights reserved.