University Of Tasmania
128291 - Thread based electrofluidic platform for direct metabolite analysis in complex samples - Post print.pdf (914.01 kB)
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Thread based electrofluidic platform for direct metabolite analysis in complex samples

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journal contribution
posted on 2023-05-19, 21:13 authored by Cabot, JM, Michael BreadmoreMichael Breadmore, Brett PaullBrett Paull
The application of electrophoresis upon commercial threads is investigated for development of low-cost diagnostics assays, designed for the matrix separation and quantification of low abundance metabolites in complex samples – in this work riboflavin in human urine. Zone electrophoresis was evaluated upon 8 commercially available threads, with several synthetic threads exhibiting higher electroosmotic flow (EOF) and increased electrophoretic mobility of the rhodamine 6G, rhodamine B, and fluorescein. Of those tested, a nylon bundle was selected as the best platform, offering less band dispersion and higher resolution, a high relative EOF, whilst minimising the contribution of joule heating. A novel 3D printed platform was designed, based on a modular system, facilitating the electrophoresis process and rapid assembly, whilst offering the potential for multiplexed analysis or investigation of more complex systems. Using the thread-based electrophoresis system, riboflavin was determined in less than 2 minutes. The device exhibited a linear working range from 0.1 to 15 µg/mL of riboflavin in urine, and was in good agreement with capillary electrophoresis measurements.


Australian Research Council


Publication title

Analytica Chimica Acta








School of Natural Sciences


Elsevier Science Bv

Place of publication

Po Box 211, Amsterdam, Netherlands, 1000 Ae

Rights statement

Copyright 2017 Elsevier B.V.

Repository Status

  • Open

Socio-economic Objectives

Expanding knowledge in the chemical sciences