University Of Tasmania
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Transcription of the interferon gamma (IFN-gamma )-inducible chemokine Mig in IFN-gamma-deficient mice

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journal contribution
posted on 2023-05-20, 21:02 authored by Mahalingam, S, Chaudhri, G, Tan, CL, John, A, Foster, PS, Gunasegaran KarupiahGunasegaran Karupiah
MuMig or Mig (murine monokine induced by interferon gamma) is a CXC chemokine whose induction is thought to be strictly dependent on interferon gamma (IFN-gamma). Here we have studied the expression of this chemokine gene in various organs of mice infected with vaccinia virus. We have employed animals deficient in either IFN-gamma (IFN-gamma(-/-)), or receptors for IFN-alpha/beta, IFN-gamma, or both IFN-alpha/beta and IFN-gamma (DR(-/-)) to dissect out the role of interferons in the induction of Mig during the host response to virus infection. Our data show that Mig mRNA and protein are expressed in organs of vaccinia virus-infected IFN-gamma(-/-) mice, albeit at lower levels compared with infected, wild-type animals. In the DR(-/-) mice and in IFN-gamma(-/-) mice treated with a neutralizing antibody to IFN-alpha/beta, Mig mRNA transcripts were completely absent. Our data indicate that, in vaccinia virus-infected IFN-gamma(-/-) mice, Mig mRNA expression is mediated through the interaction between IFN-gamma responsive element 1 (gammaRE-1) and IFN-alpha/beta-induced STAT-1 complex referred to as IFN-gamma response factor 2 (gammaRF-2). Further, our findings support the view that gammaRF-2 is the IFN-alpha/beta induced STAT-1 complex, IFN-alpha-activated factor. We have found that, in the absence of IFN-gamma, IFN-alpha/beta are able to induce Mig in response to a viral infection in vivo.


Publication title

Journal of Biological Chemistry










Tasmanian School of Medicine


Amer Soc Biochemistry Molecular Biology Inc

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9650 Rockville Pike, Bethesda, USA, Md, 20814-3996

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© 2004 by The American Society for Biochemistry and Molecular Biology, Inc. This is an Open Access article under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. (

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  • Open

Socio-economic Objectives

Prevention of human diseases and conditions; Treatment of human diseases and conditions