University of Tasmania
Wound_skin_genexp_2016.pdf (2.06 MB)

Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB

Download (2.06 MB)
journal contribution
posted on 2023-05-20, 18:51 authored by Haustead, HJ, Stevenson, A, Saxena, V, Marriage, F, Firth, M, Silla, R, Martin, L, Adcroft, KF, Rea, S, Day, PJ, Phillip MeltonPhillip Melton, Wood, FM, Fear, MW
Age is well-known to be a significant factor in both disease pathology and response to treatment, yet the molecular changes that occur with age in humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate that there is a period of significantly elevated, transcriptome-wide expression changes occurring predominantly in middle age. Both pre and post this period, the transcriptome appears to undergo much smaller, linear changes with increasing age. Functional analysis of the transient changes in middle age suggest a period of heightened metabolic activity and cellular damage associated with NF-kappa-B and TNF signaling pathways. Through metaanalysis we also show the presence of global, tissue independent linear transcriptome changes with age which appear to be regulated by NF-kappa-B. These results suggest that aging in human skin is associated with a critical mid-life period with widespread transcriptome changes, both preceded and proceeded by a relatively steady rate of linear change in the transcriptome. The data provides insight into molecular changes associated with normal aging and will help to better understand the increasingly important pathological changes associated with aging.


Publication title

Scientific Reports








Menzies Institute for Medical Research


Nature Publishing Group

Place of publication

United Kingdom

Rights statement

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit

Repository Status

  • Open

Socio-economic Objectives

Clinical health not elsewhere classified

Usage metrics

    University Of Tasmania


    Ref. manager