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UVB-induced melanocyte proliferation in neonatal mice driven by CCR2-independent recruitment of Ly6clow MHCIIhi macrophages

journal contribution
posted on 2023-05-17, 21:59 authored by Handoko, HY, Rodero, MP, Boyle, GM, Ferguson, B, Engwerda, C, Hill, G, Muller, HK, Khosrotehrani, K, Walker, GJ
Intermittent sunburns, particularly in childhood, are the strongest environmental risk factor for malignant melanoma (MM). In mice, a single neonatal UVR exposure induces MM, whereas chronic doses to adult mice do not. Neonatal UVR alters melanocyte migration dynamics by inducing their movement upward out of hair follicles into the epidermis. UVR is known to induce inflammation and recruitment of macrophages into the skin. In this study, we have used a liposomal clodronate strategy to deplete macrophages at the time of neonatal UVR, and have shown functionally that this reduces the melanocyte proliferative response. This effect was not reproduced by depletion of CD11c-expressing populations of dendritic cells. On the basis of epidermal expression array data at various time points after UVR, we selected mouse strains defective in various aspects of macrophage recruitment, activation, and effector functions, and measured their melanocyte UVR response. We identified Ly6c low MHCII hi macrophages as the major population promoting the melanocyte response across multiple strains. The activity of this subpopulation was CCR2 (C-C chemokine receptor type 2) independent and partly IL-17 dependent. By helping induce this effect, the infiltration of specific macrophage subpopulations after sunburn may be a factor in increasing the risk of subsequent neoplastic transformation of melanocytes. © 2013 The Society for Investigative Dermatology.


Publication title

Journal of Investigative Dermatology










Tasmanian School of Medicine


Nature Publishing Group

Place of publication

United Kingdom

Rights statement

Copyright 2013 The Society for Investigative Dermatology

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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