University of Tasmania
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Ultrastructural in situ hybridization and autoradiographic detection of foreign DNA in zebrafish spermatozoa

journal contribution
posted on 2023-05-18, 04:49 authored by Jawahar PATILJawahar PATIL, Khoo, HW
Conditions have been established which permit specific hybridization of foreign DNA to complimentary DNA in ultra thin sections of zebrafish sperm prepared for electron microscopy (EM) after fixation in 2% paraformaldehyde, 2.5% glutaraldehyde and embedded in spurs resin. Zebrafish sperm incubated with foreign DNA were subjected to ultrastructural in situ hybridization and autoradiographic detection to determine if the sperm could retain and internalize the foreign DNA sequences. Specific and intense signals were detected from the sperm head indicating that the zebrafish sperm are capable of internalizing foreign DNA.

History

Publication title

Biochemistry and Molecular Biology International

Volume

35

Issue

5

Pagination

965-969

ISSN

1039-9712

Department/School

Institute for Marine and Antarctic Studies

Publisher

Wiley-Blackwell Publishing, Inc.

Place of publication

Australia

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences