University Of Tasmania
Browse

File(s) under permanent embargo

Understanding the mechanistic basis of ameliorating effects of hydrogen rich water on salinity tolerance in barley

journal contribution
posted on 2023-05-21, 04:00 authored by Wu, Qi, Su, N, Svetlana ShabalaSvetlana Shabala, Huang, L, Yu, M, Sergey ShabalaSergey Shabala
Hydrogen gas (H2) is known for its capability to alleviate detrimental effects of salinity in plants. However, the underlying physiological and molecular mechanisms behind this phenomenon remain poorly understood. In this study, we investigated the mechanistic basis of H2 amelioration of the salt stress in barley (Hordeum vulgare). NaCl addition induced significant inhibition of the root elongation and resulted in a loss of the cell viability; these detrimental effects were substantially reversed by treatment with hydrogen reach water (HRW). Electrophysiological experiments using non-invasive ion flux measuring MIFE technique revealed that the beneficial effects of HRW on salinity tolerance could be explained by (1) higher rate of Na+ extrusion from roots mediated by SOS1-like Na+/H+ exchanger in the root epidermis, and (2) better root K+ retention resulting from ability of HRW-treated plants to prevent NaCl-induced membrane depolarization and reduced sensitivity of K+ efflux channels to ROS. Taken together, these two factors resulted in more favorable Na/K ratio and explained beneficial effects of HRW on salinity tolerance in barley.

History

Publication title

Environmental and Experimental Botany

Volume

177

Article number

104136

Number

104136

ISSN

0098-8472

Department/School

School of Natural Sciences

Publisher

Pergamon-Elsevier Science Ltd

Place of publication

The Boulevard, Langford Lane, Kidlington, Oxford, England, Ox5 1Gb

Rights statement

Copyright 2020 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Other plant production and plant primary products not elsewhere classified