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Urea output by L3 Teladorsagia circumcincta and some properties of two urea producing enzymes
Background: Like several other parasites, Teladorsagia circumcincta secretes or excretes urea, but neither the rate of efflux nor the possible metabolic sources of the urea has been considered.
Methods: Parasites were maintained by passage through sheep. Urea efflux was measured using phenol/hypochlorite after treatment with urea aminohydrolase. The kinetics of creatine amidinohydrolase and arginine amidinohydrolase were characterised by coupling the reactions with urea aminohydrolase and glutamate dehydrogenase.
Results: Infective L3 T. circumcincta secreted or excreted urea at 25% of the rate of NH3/NH4+. The rate of urea efflux was about 84 pmol h-1 (103 larvae)-1 over 4 hours, corresponding to about 11 nmol h-1 mg-1 protein. We could not detect urea aminohydrolase activity, but urea production by both creatine amidinohydrolase and arginine amidinohydrolase could be detected. The apparent Km and Vmax of creatine amidinohydrolase were 1.1 mM and 48 nmol h-1 mg-1 protein, respectively, and the activity was greatest at pH 8. The apparent Km and Vmax of arginine amidinohydrolase were 0.7 mM and 62 nmol h-1 mg-1 protein, respectively, and the activity was greatest at pH 7.9.
Conclusion: The activity of creatine amidinohydrolase and arginine amidinohydrolase was sufficient to account for the rate of urea secretion or excretion.
Publication titleIranian Journal of Parasitology
Department/SchoolTasmanian School of Medicine
PublisherTehran University of Medical Sciences Publications
Place of publicationIran
Rights statementCopyright 2013 Iranian Journal of Parasitology