Using time-resolved fluorescence to measure serum venom-specific IgE and IgG
journal contributionposted on 2023-05-19, 08:36 authored by van Eeden, PE, Wiese, MD, Aulfrey, S, Hales, BJ, Stone, SF, Brown, SG
We adapted DELFIAâ„¢ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG1 and sIgG4 in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intraassay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms. Â© 2011 van Eeden et al.
Publication titlePLoS ONE
Department/SchoolTasmanian School of Medicine
PublisherPublic Library of Science
Place of publicationUnited States
Rights statementCopyright 2011 van Eeden et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited