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Utility of gel-free, label-free shotgun proteomics approaches to investigate microorganisms
journal contribution
posted on 2023-05-17, 05:39 authored by Porteus, B, Chawalit KocharunchittChawalit Kocharunchitt, Nilsson, RE, Thomas RossThomas Ross, John BowmanJohn BowmanThis review will examine the current situation with label-free, quantitative, shotgun-oriented proteomics technology and discuss the advantages and limitations associated with its capability in capturing and quantifying large portions of proteomes of microorganisms. Such an approach allows (1) comparisons between physiological or genetic states of organisms at the protein level, (2) ‘painting’ of proteomic data onto genome data-based metabolic maps, (3) enhancement of the utility of genomic data and finally (4) surveying of non-genome sequenced microorganisms by taking advantage of available inferred protein data in order to gain new insights into straindependent metabolic or physiological capacities. The technology essentially is a powerful addition to systems biology with a capacity to be used to ask hypothesis-driven ‘top-down’ questions or for more empirical ‘bottom-up’ exploration.
Funding
Meat and Livestock Australia
History
Publication title
Applied Microbiology and BiotechnologyVolume
90Pagination
407-416ISSN
0175-7598Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Springer-VerlagPlace of publication
175 Fifth Ave, New York, USA, Ny, 10010Rights statement
The final publication is available at http://www.springerlink.comRepository Status
- Restricted