University Of Tasmania
154575 - Morris et al 2023 J Neurosci Res.pdf (4.16 MB)

Vascular perfusion differs in two distinct PDGFRβ-positive zones within the ischemic core of male mice 2 weeks following photothrombotic stroke

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journal contribution
posted on 2023-05-21, 15:19 authored by Gary MorrisGary Morris, Gowing, EK, Jo-Maree CourtneyJo-Maree Courtney, Hannah CoombeHannah Coombe, Natalie KingNatalie King, Rewell, SSJ, David Howells, Clarkson, AN, Brad SutherlandBrad Sutherland
Stroke therapy has largely focused on preventing damage and encouraging repair outside the ischemic core, as the core is considered irreparable. Recently, several studies have suggested endogenous responses within the core are important for limiting the spread of damage and enhancing recovery, but the role of blood flow and capillary pericytes in this process is unknown. Using the Rose Bengal photothrombotic model of stroke, we illustrate blood vessels are present in the ischemic core and peri-lesional regions 2 weeks post stroke in male mice. A FITC-albumin gel cast of the vasculature revealed perfusion of these vessels, suggesting cerebral blood flow (CBF) may be partially present, without vascular leakage. The length of these vessels is significantly reduced compared to uninjured regions, but the average width is greater, suggesting they are either larger vessels that survived the initial injury, smaller vessels that have expanded in size (i.e., arteriogenesis), or that neovascularization begins with larger vessels. Concurrently, we observed an increase in platelet-derived growth factor receptor beta (PDGFRβ, a marker of pericytes) expression within the ischemic core in two distinct patterns, one which resembles pericyte-derived fibrotic scarring at the edge of the core, and one which is vessel associated and may represent blood vessel recovery. We find little evidence for dividing cells on these intralesional blood vessels 2 weeks post stroke. Our study provides evidence flow is present in PDGFRβ-positive vessels in the ischemic core 2 weeks post stroke. We hypothesize intralesional CBF is important for limiting injury and for encouraging endogenous repair following cerebral ischemia.


National Health & Medical Research Council


Publication title

Journal of neuroscience research



Article number







Tasmanian School of Medicine


Wiley Interscience

Place of publication

New York

Rights statement

Copyright (2023) The Authors. This is an open access article under the Creative Commons Attribution-NonCommercial License (

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  • Open

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