Zero net-flow in capillary electrophoresis using acrylamide based hydrogel

Zero net-flow was observed when acrylamide based hydrogel was used in a vial at one end of a fused-silica capillary during electrophoresis with electroosmotic flow. We demonstrate the detection of anionic compounds with the anode at the detector end and the field-enhanced sample injection of anionic small molecule drugs in counter-electroosmotic flow capillary zone electrophoresis.