whole_RobertsKylieMaree2009_thesis.pdf (26.43 MB)
Proteomic analysis of breast cancer
thesisposted on 2023-05-27, 17:17 authored by Roberts, Kylie Maree
Introduction: Breast cancer is a disease that will affect every woman in her life. Whether that affect will be direct or indirect is, at this stage almost impossible to determine. There have been several key findings in recent years with genes being found that are linked directly to breast cancer. With these being attributed to less than 10% of breast cancers diagnosed, the need for new biomarkers of breast cancer exists. This study aims to find new biomarkers though differential analysis of breast cancer tissue. Several different analysis have been conducted to provide insight in to potential new markers for diagnosing early onset breast cancers, looking for signs in otherwise healthy tissue and ascertaining differences between cancer and healthy tissue as a whole. This study also looks in to the functional areas of proteomic research, determining a need for further investigation in to IGF-1 and ~-casein, which have been inconclusively linked to breast cancer in the past. Methods: By employing selected proteomic technologies including iso-electric focusing and polyacrylamide gel electrophoresis and combining samples from populations from different locations across the globe, collections of two-dimensional gels from breast tissue were differentially compared to isolate proteins that have a high likelihood of being involved in breast cancer. Results: This approach to breast cancer research has led to many proteins being isolated that have a role in breast cancer. The proteins found have a diverse range of roles from signaling proteins to structural proteins. Within those proteins successfully identified are proteins like HSP60, known to have a role in breast cancer, serum albumin which has limited information regarding a role in breast cancer and FLJ20309, one of several proteins that have been assumed from cDNA clones with little available information available regarding its function. Conclusions: This study has led to the discovery of proteins that were previously thought to have limited, if no input in breast cancer. All of the proteins found require further experiments to fully elucidate their function in breast cancer however at least some of these are show promise as being of diagnostic and/or therapeutic value.
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