Wu_whole_thesis_ex_pub_mat.pdf (6.82 MB)
Tissue specificity of cytosolic `K^+` retention, `Na^+` extrusion, and vacuolar `Na^+` sequestration traits in the context of differential salinity stress tolerance in barley and wheat
thesisposted on 2023-05-27, 10:12 authored by Wu, H
Wheat and barley are ranked the second and fifth most important crops in terms of dry matter production. Both of them are classified as glycophytes, and their production is strongly affected by soil salinity. Thus, given the extent of land salinization in the world and predicted population growth to 9.3 billion by 2050, creating salt tolerant wheat and barley germplasm remains one of highest priorities for breeders. Salinity tolerance is a complex physiological trait composed of numerous sub-traits controlled by multiple regulatory pathways. Until now, most studies were focused on traits related to sodium, such as `Na^+` exclusion from uptake, control of xylem `Na^+` loading, `Na^+` retrieval from the shoot or vacuolar Na+ sequestration. However, it is not `Na^+` but the `K^+``/Na^+` ratio in the cytosol that ultimately determines plant performance under saline conditions. In recent years, `K^+` retention in root mature zone has emerged as an important component of salt tolerance mechanisms in many plant species. However, whether the importance of cell's ability to maintain `K^+` in plant overall salt tolerance can be extrapolated to other root zones or tissues (e.g. leaves) remained obscure prior to this work. Also elusive remained the essentiality of root `Na^+` exclusion and vacuolar `Na^+` sequestration in various root tissues. Furthermore, the relative contribution of each of the above salt tolerant mechanisms towards the overall salinity tolerance remained unclear, especially at the tissue specific level.
Rights statementCopyright 2015 the Author Chapter 3 appears to be the equivalent of the peer reviewed version of the following article: Wu, H., Shabala, L., Barry, K., Zhou, M., Shabala, S., (2013). Ability of leaf mesophyll to retain potassium correlates with salinity tolerance in wheat and barley, Physiologia plantarum 149(4), 515‚Äö-527, which has been published in final form at http://dx.doi.org/10.1111/ppl.12056I This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Chapter 4 appears to be the equivalent of the peer reviewed version of the following article: Wu, H., Shabala, L., Zhou, M., Shabala, S., (2015). `K^+` retention in leaf mesophyll, an overlooked component of salinity tolerance mechanism: a case for barley, Journal of integrative plant biology, 57(2), 171‚Äö-185, which has been published in final form at http://dx.doi.org/10.1111/jipb.12238 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Chapter 5 appears to be the equivalent of a pre-copyedited, author-produced version of an article accepted for publication in Plant and cell physiology following peer review. The version of record, Wu. H., Shabala, L., Zhou, M., Shabala, S. (2014). Durum and bread wheat differ in their ability to retain potassium in leaf mesophyll: implications for salinity stress tolerance, Plant and cell physiology, 55(10), 1749‚Äö-1762, is available online at: https://doi.org/10.1093/pcp/pcu105 Chapter 6 appears to be the equivalent of a post-print version of an article published as: Wu, H., Shabala, L., Zhou, M., Stefano, G., Pandolfi, C., Mancuso, S., Shabala, S., (2015). Developing and validating a high-throughput assay for salinity tissue tolerance in wheat and barley, Planta, 242(4), 847‚Äö-857. The final publication is available at Springer via http://dx.doi.org/10.1007/s00425-015-2317-1 Chapter 7 appears to be the equivalent of a post-print version of an article published as: Wu, H., Shabala, L., Liu, X., Azzarello, E., Zhou, M., Pandolfi, C., Zhong-Hua, C., Bose, J., Mancuso, S., Shabala, S., (2015). Linking salinity stress tolerance with tissue-specific `Na^+` sequestration in wheat roots, Frontiers in plant science, 6, article 71, 1-13. This Document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission. Appendix 1 has been published as: Wu, H., Shabala, L., Zhou, M., Shabala, S., (2015). MIFE technique-based screening for mesophyll `K^+` retention for crop breeding for salinity tolerance, Bio-protocol 5(9), e1466, 1-10. It has been removed for copyright or proprietary reasons. Appendix 2 appears to be the equivalent of an accepted manuscript of an article published by Taylor & Francis in Plant signaling and behavior on 3 June 2015, available online: http://www.tandfonline.com/doi/full/10.1080/15592324.2015.1013793