University Of Tasmania
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Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect

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journal contribution
posted on 2023-05-20, 22:17 authored by Chuang, Y-F, Andrew PhippsAndrew Phipps, Fan-Li Lin, Valerie HechtValerie Hecht, Alexander HewittAlexander Hewitt, Wang, P-Y, Guei-Sheung LiuGuei-Sheung Liu
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system provides a groundbreaking genetic technology that allows scientists to modify genes by targeting specific genomic sites. Due to the relative simplicity and versatility of the CRISPR/Cas system, it has been extensively applied in human genetic research as well as in agricultural applications, such as improving crops. Since the gene editing activity of the CRISPR/Cas system largely depends on the efficiency of introducing the system into cells or tissues, an efficient and specific delivery system is critical for applying CRISPR/Cas technology. However, there are still some hurdles remaining for the translatability of CRISPR/Cas system. In this review, we summarized the approaches used for the delivery of the CRISPR/Cas system in mammals, plants, and aquacultures. We further discussed the aspects of delivery that can be improved to elevate the potential for CRISPR/Cas translatability.


National Health & Medical Research Council


Publication title

Cellular and Molecular Life Sciences










Menzies Institute for Medical Research


Birkhauser Verlag Ag

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© The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature 2021

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  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences