138059 - Iron is increased in the brains of ageing mice.pdf (892.79 kB)
Download fileIron is increased in the brains of ageing mice lacking the neurofilament light gene
journal contribution
posted on 2023-05-20, 11:58 authored by James VickersJames Vickers, Anna KingAnna King, Graeme McCormackGraeme McCormack, Aidan BindoffAidan Bindoff, Adlard, PAThere has been strong interest in the role of metals in neurodegeneration, and how ageing may predispose the brain to related diseases such as Alzheimer's disease. Recent work has also highlighted a potential interaction between different metal species and various components of the cytoskeletal network in the brain, which themselves have a reported role in age-related degenerative disease and other neurological disorders. Neurofilaments are one such class of intermediate filament protein that have a demonstrated capacity to bind and utilise cation species. In this study, we investigated the consequences of altering the neurofilamentous network on metal ion homeostasis by examining neurofilament light (NFL) gene knockout mice, relative to wildtype control animals, at adulthood (5 months of age) and advanced age (22 months). Inductively coupled plasma mass spectroscopy demonstrated that the concentrations of iron (Fe), copper (Cu) and zinc (Zn) varied across brain regions and peripheral nerve samples. Zn and Fe showed statistically significant interactions between genotype and age, as well as between genotype and region, and Cu demonstrated a genotype and region interaction. The most substantial difference between genotypes was found in Fe in the older animals, where, across many regions examined, there was elevated Fe in the NFL knockout mice. This data indicates a potential relationship between the neurofilamentous cytoskeleton and the processing and/or storage of Fe through ageing.
History
Publication title
PLoS ONEVolume
14Issue
10Article number
e0224169Number
e0224169Pagination
1-9ISSN
1932-6203Department/School
Wicking Dementia Research Education CentrePublisher
Public Library of SciencePlace of publication
United StatesRights statement
Copyright 2019 Vickers et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/Repository Status
- Open