University of Tasmania
Foo et al 2020_MRM_Accepted Manuscript.pdf (2.18 MB)

Study of common quantification methods of amide proton transfer magnetic resonance imaging for ischemic stroke detection

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journal contribution
posted on 2023-05-20, 19:01 authored by Foo, LS, Larkin, JR, Brad SutherlandBrad Sutherland, Ray, KJ, Yap, W-S, Hum, YC, Lai, KW, Manan, HA, Sibson, NR, Tee, YK

Purpose: To assess the correlation and differences between common amide proton transfer (APT) quantification methods in the diagnosis of ischemic stroke.

Methods: Five APT quantification methods, including asymmetry analysis and its variants as well as two Lorentzian model-based methods, were applied to data acquired from six rats that underwent middle cerebral artery occlusion scanned at 9.4T. Diffusion and perfusion-weighted images, and water relaxation time maps were also acquired to study the relationship of these conventional imaging modalities with the different APT quantification methods.

Results: The APT ischemic area estimates had varying sizes (Jaccard index: 0.544 ≤ J ≤ 0.971) and had varying correlations in their distributions (Pearson correlation coefficient: 0.104 ≤ r ≤ 0.995), revealing discrepancies in the quantified ischemic areas. The Lorentzian methods produced the highest contrast-to-noise ratios (CNRs; 1.427 ≤ CNR ≤ 2.002), but generated APT ischemic areas that were comparable in size to the cerebral blood flow (CBF) deficit areas; asymmetry analysis and its variants produced APT ischemic areas that were smaller than the CBF deficit areas but larger than the apparent diffusion coefficient deficit areas, though having lower CNRs (0.561 ≤ CNR ≤ 1.083).

Conclusion: There is a need to further investigate the accuracy and correlation of each quantification method with the pathophysiology using a larger scale multi-imaging modality and multi-time-point clinical study. Future studies should include the magnetization transfer ratio asymmetry results alongside the findings of the study to facilitate the comparison of results between different centers and also the published literature.


National Health & Medical Research Council


Publication title

Magnetic Resonance in Medicine










Tasmanian School of Medicine


John Wiley & Sons Inc

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111 River St, Hoboken, USA, Nj, 07030

Rights statement

© 2020 International Society for Magnetic Resonance in Medicine This is the peer reviewed version of the following article: Foo LS, Larkin JR, Sutherland BA, et al. Study of common quantification methods of amide proton transfer magnetic resonance imaging for ischemic stroke detection. Magn Reson Med. 2020;00:1–13., which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

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